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green fluorescent dna marker dye  (Beyotime)


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    Structured Review

    Beyotime green fluorescent dna marker dye
    a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP <t>DNA</t> segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green <t>fluorescent</t> protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.
    Green Fluorescent Dna Marker Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/green+fluorescent+dna+marker+dye/InstantView+Green+Fluorescent+DNA+Marker+Dye/pmc11164925-198-21-37
    Average 99 stars, based on 1 article reviews
    green fluorescent dna marker dye - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry"

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry

    Journal: Nature Communications

    doi: 10.1038/s41467-024-49412-9

    a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP DNA segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green fluorescent protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.
    Figure Legend Snippet: a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP DNA segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green fluorescent protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.

    Techniques Used: Irradiation, Recombinant

    a Schematic illustration of two parC -eGFP DNAs being pushed through ParM polymerization in a giant unilamellar vesicles (GUV) and subsequent GUV division under laser irradiation. b Confocal microscopy images of GUV division ( b1 – b3 ) and enhanced green fluorescent protein (eGFP) expression at 37 °C ( b4 – b7 ). b1 – b3 indicate GUV deformation, filament splitting, and division into two daughter cells, respectively. The white arrows in b1 and b2 indicate parC -eGFP DNA. The ParM filament was split by laser irradiation (561 nm, 0.7 mW, 5 s). The scale bars are 10 μm. n = 3 independent replicates. c Schematic illustration of eGFP expression in two daughter cells. The corresponding fluorescence intensity (FI) in daughter GUV 1 ( d ) and daughter GUV 2 ( e ) as a function of time. The normalized fluorescence intensity of eGFP was calculated from three independent samples. The data were presented as the mean values ± SDs; n = 3 independent replicates. Source data are provided as a Source Data file.
    Figure Legend Snippet: a Schematic illustration of two parC -eGFP DNAs being pushed through ParM polymerization in a giant unilamellar vesicles (GUV) and subsequent GUV division under laser irradiation. b Confocal microscopy images of GUV division ( b1 – b3 ) and enhanced green fluorescent protein (eGFP) expression at 37 °C ( b4 – b7 ). b1 – b3 indicate GUV deformation, filament splitting, and division into two daughter cells, respectively. The white arrows in b1 and b2 indicate parC -eGFP DNA. The ParM filament was split by laser irradiation (561 nm, 0.7 mW, 5 s). The scale bars are 10 μm. n = 3 independent replicates. c Schematic illustration of eGFP expression in two daughter cells. The corresponding fluorescence intensity (FI) in daughter GUV 1 ( d ) and daughter GUV 2 ( e ) as a function of time. The normalized fluorescence intensity of eGFP was calculated from three independent samples. The data were presented as the mean values ± SDs; n = 3 independent replicates. Source data are provided as a Source Data file.

    Techniques Used: Irradiation, Confocal Microscopy, Expressing, Fluorescence

    Related Articles

    Bradford Protein Assay:

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry
    Article Snippet: Cy3 maleimide (nonsulfonated) was purchased from APExBIO (USA). .. A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China). .. Protein markers (14.4–-97.4 kDa), 2×SYBR Green PCR Master Mix, a Na + K + -ATPase assay kit, TE buffer (pH 8.0), deoxyribonuclease I (DNase I), deoxyribonucleic acid sodium salt from salmon testes, and dithiothreitol (DTT) were obtained from Solarbio (China).

    Protease Inhibitor:

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry
    Article Snippet: Cy3 maleimide (nonsulfonated) was purchased from APExBIO (USA). .. A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China). .. Protein markers (14.4–-97.4 kDa), 2×SYBR Green PCR Master Mix, a Na + K + -ATPase assay kit, TE buffer (pH 8.0), deoxyribonuclease I (DNase I), deoxyribonucleic acid sodium salt from salmon testes, and dithiothreitol (DTT) were obtained from Solarbio (China).

    Purification:

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry
    Article Snippet: Cy3 maleimide (nonsulfonated) was purchased from APExBIO (USA). .. A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China). .. Protein markers (14.4–-97.4 kDa), 2×SYBR Green PCR Master Mix, a Na + K + -ATPase assay kit, TE buffer (pH 8.0), deoxyribonuclease I (DNase I), deoxyribonucleic acid sodium salt from salmon testes, and dithiothreitol (DTT) were obtained from Solarbio (China).

    Marker:

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry
    Article Snippet: Cy3 maleimide (nonsulfonated) was purchased from APExBIO (USA). .. A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China). .. Protein markers (14.4–-97.4 kDa), 2×SYBR Green PCR Master Mix, a Na + K + -ATPase assay kit, TE buffer (pH 8.0), deoxyribonuclease I (DNase I), deoxyribonucleic acid sodium salt from salmon testes, and dithiothreitol (DTT) were obtained from Solarbio (China).

    Magnetic Beads:

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry
    Article Snippet: Cy3 maleimide (nonsulfonated) was purchased from APExBIO (USA). .. A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China). .. Protein markers (14.4–-97.4 kDa), 2×SYBR Green PCR Master Mix, a Na + K + -ATPase assay kit, TE buffer (pH 8.0), deoxyribonuclease I (DNase I), deoxyribonucleic acid sodium salt from salmon testes, and dithiothreitol (DTT) were obtained from Solarbio (China).



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    a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP <t>DNA</t> segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green <t>fluorescent</t> protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.
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    a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP <t>DNA</t> segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green <t>fluorescent</t> protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.
    Instantview Green Fluorescent Dna Marker Dye D0153, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP DNA segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green fluorescent protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.

    Journal: Nature Communications

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry

    doi: 10.1038/s41467-024-49412-9

    Figure Lengend Snippet: a Giant unilamellar vesicles (GUV) containing the ParMRC system and PURE system. b parC -eGFP DNA segregation by the polymerization of ParM triggered by adenosine triphosphate (ATP) influx upon laser irradiation. c A deformed GUV under hypertonic conditions. d Two daughter GUVs containing parC -eGFP DNA and the PURE system upon laser irradiation at the center region of the deformed GUV (purple area in Fig. 1c). e Enhanced green fluorescent protein (eGFP) was expressed inside two daughter GUVs through translating the eGFP gene using a PURE (protein synthesis using recombinant elements) system at 37 °C. The PURE system contains ribosomes, amino acids, nucleoside triphosphates (NTPs), transfer ribonucleic acid (tRNAs), enzyme substrates, RNA polymerase, translation factors, and other necessary components.

    Article Snippet: A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China).

    Techniques: Irradiation, Recombinant

    a Schematic illustration of two parC -eGFP DNAs being pushed through ParM polymerization in a giant unilamellar vesicles (GUV) and subsequent GUV division under laser irradiation. b Confocal microscopy images of GUV division ( b1 – b3 ) and enhanced green fluorescent protein (eGFP) expression at 37 °C ( b4 – b7 ). b1 – b3 indicate GUV deformation, filament splitting, and division into two daughter cells, respectively. The white arrows in b1 and b2 indicate parC -eGFP DNA. The ParM filament was split by laser irradiation (561 nm, 0.7 mW, 5 s). The scale bars are 10 μm. n = 3 independent replicates. c Schematic illustration of eGFP expression in two daughter cells. The corresponding fluorescence intensity (FI) in daughter GUV 1 ( d ) and daughter GUV 2 ( e ) as a function of time. The normalized fluorescence intensity of eGFP was calculated from three independent samples. The data were presented as the mean values ± SDs; n = 3 independent replicates. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry

    doi: 10.1038/s41467-024-49412-9

    Figure Lengend Snippet: a Schematic illustration of two parC -eGFP DNAs being pushed through ParM polymerization in a giant unilamellar vesicles (GUV) and subsequent GUV division under laser irradiation. b Confocal microscopy images of GUV division ( b1 – b3 ) and enhanced green fluorescent protein (eGFP) expression at 37 °C ( b4 – b7 ). b1 – b3 indicate GUV deformation, filament splitting, and division into two daughter cells, respectively. The white arrows in b1 and b2 indicate parC -eGFP DNA. The ParM filament was split by laser irradiation (561 nm, 0.7 mW, 5 s). The scale bars are 10 μm. n = 3 independent replicates. c Schematic illustration of eGFP expression in two daughter cells. The corresponding fluorescence intensity (FI) in daughter GUV 1 ( d ) and daughter GUV 2 ( e ) as a function of time. The normalized fluorescence intensity of eGFP was calculated from three independent samples. The data were presented as the mean values ± SDs; n = 3 independent replicates. Source data are provided as a Source Data file.

    Article Snippet: A Bradford protein assay kit, SDS‒PAGE gel configuration kit, 4×SDS‒PAGE sample loading buffer, protease inhibitor cocktail for purification of His-tagged proteins, green fluorescent DNA marker dye, streptavidin magnetic beads, Tris-borate-EDTA buffer (TBE) and Tween-20 were purchased from Beyotime (China).

    Techniques: Irradiation, Confocal Microscopy, Expressing, Fluorescence